Molecular Formula | C4H11Cl2N2O2P |
Molar Mass | 221.02 |
Density | 1.474±0.06 g/cm3(Predicted) |
Melting Point | 115 °C |
Boling Point | 363.5±52.0 °C(Predicted) |
pKa | 0.41±0.50(Predicted) |
Toxicity | LD50 intraperitoneal in mouse: 184mg/kg |
biological activity | Phosphoramide mudard is an active metabolite of cyclophosphamide (HY-17420) and has antitumor activity. Phosphoramide mustard can induce DNA damage. |
Target | DNA Alkylator |
Cell Line: | SIGCs SIGCs SIGCs |
Concentration: | 0.5 μM, 1 μM, 3 μM, 6 μM 3 μM, 6 μM 3 μM, 6 μM |
Incubation Time: | 48 hours 24 hours, 48 hours 24 hours, 48 hours |
Result: | Reduced cell viability at concentrations of 3 μM and higher. Increased DDR gene mRNA expression levels. Generally increased DDR proteins. Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg. T 1/2 (15.1 min). |
Animal Model: | Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor Rats |
Dosage: | 2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg 59.4 mg/kg (Pharmacokinetic Analysis) |
Administration: | Intraperitoneal injection, once daily, for 5 consecutive days Intravenous injection |